Start-up ecosystems have been responsible for enhancing innovation, employment and economic development across many developing regions world-wide; particularly where there are geographical obstacles to development. This study aims to examine how the start-up ecosystem can support the region of Sikkim, India with its emerging ecosystem to enhance sustainable economic growth. The primary data collected from 212 entrepreneurs across all districts in Sikkim using structured questionnaire will be analysed by statistical tools such as descriptive statistics, One-sample t-test, analysis of variance (ANOVA) and relative importance index (RII). From the outcome it is observed that government support, digital connectivity, tourism potential and local entrepreneurship initiatives positively contribute to develop the start-up ecosystem of Sikkim. However, challenges such as limited access to funds, lack of infrastructure, market accessibility issues and skill gap continue to limit entrepreneurial growth. The study has also pointed out that policy implementation must be strengthened, incubation support should be improved, financial accessibility should be facilitated and entrepreneurship development programmes should be provided to promote sustainable economic development in the region.